The potential of metatranscriptomics for identifying screening targets for bacterial vaginosis
Background The ribosomal RNA content of a sample collected from a woman with bacterial
vaginosis (BV) was analysed to determine the active microbial community, and to identify
potential targets for further screening. Methodology/Principal Findings The sample from the
BV patient underwent total RNA extraction, followed by physical subtraction of human rRNA
and whole transcriptome amplification. The metatranscriptome was sequenced using Roche
454 titanium chemistry. The bioinformatics pipeline MG-RAST and desktop DNA analysis …
vaginosis (BV) was analysed to determine the active microbial community, and to identify
potential targets for further screening. Methodology/Principal Findings The sample from the
BV patient underwent total RNA extraction, followed by physical subtraction of human rRNA
and whole transcriptome amplification. The metatranscriptome was sequenced using Roche
454 titanium chemistry. The bioinformatics pipeline MG-RAST and desktop DNA analysis …
[PDF][PDF] The Potential of Metatranscriptomics for Identifying Screening Targets for Bacterial
Background: The ribosomal RNA content of a sample collected from a woman with bacterial
vaginosis (BV) was analysed to determine the active microbial community, and to identify
potential targets for further screening. Methodology/Principal Findings: The sample from the
BV patient underwent total RNA extraction, followed by physical subtraction of human rRNA
and whole transcriptome amplification. The metatranscriptome was sequenced using Roche
454 titanium chemistry. The bioinformatics pipeline MG-RAST and desktop DNA analysis …
vaginosis (BV) was analysed to determine the active microbial community, and to identify
potential targets for further screening. Methodology/Principal Findings: The sample from the
BV patient underwent total RNA extraction, followed by physical subtraction of human rRNA
and whole transcriptome amplification. The metatranscriptome was sequenced using Roche
454 titanium chemistry. The bioinformatics pipeline MG-RAST and desktop DNA analysis …
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