faecalis conferred resistance to the bacterium against calcium hydroxide. E. faecalis A197A
was grown at 46° C until early stationary phase. Standardized bacterial suspensions were
pretreated for 1 hour either with acid-soluble collagen or acidified phosphate-buffered saline
(ac-PBS) and cultured to determine the baseline viable bacterial numbers. The bacterial
suspensions were challenged with calcium hydroxide solution. Samples were removed at 6 …