The structural assembly switch of cell division protein FtsZ probed with fluorescent allosteric inhibitors

M Artola, LB Ruíz-Avila, E Ramírez-Aportela… - Chemical …, 2017 - pubs.rsc.org
… , thus reporting open and closed FtsZ interdomain clefts. Our … the structural mechanism of
the FtsZ assembly switch, and suggest that the probes bind into the open clefts in cellular FtsZ

The search for antibacterial inhibitors targeting cell division protein ftsz at its nucleotide and allosteric binding sites

JM Andreu, S Huecas, L Araújo-Bazán… - Biomedicines, 2022 - mdpi.com
… (A) Chemical structure of fluorescent probes and allosteric … operation in solution of the FtsZ
structural assembly switch [25], … structure of SaFtsZ filaments compared to other closed FtsZ

Targeting the assembly of bacterial cell division protein FtsZ with small molecules

C Schaffner-Barbero, M Martín-Fontecha… - ACS chemical …, 2012 - ACS Publications
… for a structural activation switch built into FtsZ monomers, … by fluorescence microscopy
with FtsZ-fluorescent proteinprobes for FtsZ. On the other hand, any effective bacterial …

The structural assembly switch of cell division protein FtsZ probed with fluorescent allosteric inhibitors

ME Artola Pérez de Azanza, L Ruiz Ávila, B Laura… - 2017 - docta.ucm.es
… , thus reporting open and closed FtsZ interdomain clefts. Our … the structural mechanism of
the FtsZ assembly switch, and suggest that the probes bind into the open clefts in cellular FtsZ

Targeting the FtsZ allosteric binding site with a novel fluorescence polarization screen, cytological and structural approaches for antibacterial discovery

S Huecas, L Araújo-Bazán, FM Ruiz… - Journal of Medicinal …, 2021 - ACS Publications
… developed fluorescent probes for the interdomain cleft of FtsZ, … assembly switch in solution,
in which the side cleft between … subtilis cell division or GTP-FtsZ ring localization in our hands…

Mapping Flexibility and the Assembly Switch of Cell Division Protein FtsZ by Computational and Mutational Approaches*♦

AJ Martín-Galiano, RM Buey, M Cabezas… - Journal of Biological …, 2010 - ASBMB
… A structural change at FtsZ loop T3 was predicted by … as a fluorescence probe in the
W319Y-Y92W mutant of Mj … parts and connecting allosteric networks of the FtsZ molecule and by …

Targeting the bacterial division protein FtsZ

KA Hurley, TMA Santos, GM Nepomuceno… - Journal of medicinal …, 2016 - ACS Publications
FtsZ and the early cell division proteins localize to the … (87) Alternatively, an allosteric site
on FtsZ may modulate its … ) and the intensity of a chosen fluorescent probe (ie, 60) in seconds. …

Mutations in the bacterial cell division protein FtsZ highlight the role of GTP binding and longitudinal subunit interactions in assembly and function

HA Arjes, B Lai, E Emelue, A Steinbach, PA Levin - BMC microbiology, 2015 - Springer
… the core of FtsZ and presumably impacts the structure of the … On quantitative immunblots
probed with antisera generated … due to (1) a conformational change in the protein that alters GTP …

[PDF][PDF] The structural assembly switch of cell division protein FtsZ probed with fluorescent allosteric inhibitors

ML López-Rodrıguez, JM Andreu, S Huecas - chaconlab.org
… , thus reporting open and closed FtsZ interdomain clefts. Our … the structural mechanism of
the FtsZ assembly switch, and suggest that the probes bind into the open clefts in cellular FtsZ

A polymerization-associated structural switch in FtsZ that enables treadmilling of model filaments

JM Wagstaff, M Tsim, MA Oliva, A García-Sanchez… - MBio, 2017 - Am Soc Microbiol
FtsZ protein is a key molecule during bacterial cell division. … fluorescence microscopy studies
that during the cell division … inside subunits and has an allosteric effect on interface strength …