Purpose To evaluate the therapeutic effect of human embryonic stem cell (hESC)-derived
multipotent mesenchymal stem cells (M-MSCs) on ketamine-induced cystitis (KC) in rats.
Methods To induce KC, 10-week-old female rats were injected with 25-mg/kg ketamine
hydrochloride twice weekly for 12 weeks. In the sham group, phosphate buffered saline
(PBS) was injected instead of ketamine. One week after the final injection of ketamine, the
indicated doses (0.25, 0.5, and 1× 10 6 cells) of M-MSCs (KC+ M-MSC group) or PBS …

METHODS: To induce KC, 10-week-old female rats were given 25mg/kg ketamine
hydrochloride twice a week for 12 weeks. For the sham group, phosphate buffered saline
(PBS) was injected instead of ketamine. One week after final injection of ketamine, the
indicated dose (0.25, 0.5, and 1 x 10 6 cells) of M-MSCs (KC+ M-MSC group) or PBS vehicle
(KC group) was directly injected into the submucosal layer of the anterior wall or dome of the
bladder. One week after M-MSCs injection, the therapeutic outcomes were examined by …