Objective: To evaluate the effects of four different concentrations of Bacillus subtilis (B. subtilis) and Bacillus circulance (B. circulance)(1× 104, 2× 104, 3× 104 and 4× 104 CFU/g) on growth and resistance of three spot gourami (Trichopodus trichopterus) for a period of 30 days.
Methods: During this study, experimental fish were fed with supplemented diets. Bacillus probiotics with concentrations of 1× 104 to 4× 104 CFU/g were administered to improve the growth performance and larval resistance to challenge tests containing acidic pH and basic pH, ammonia and salinity tests.
Results: The addition of B. subtilis and B. circulance to the diet did not increase larval growth rate, but increased larval resistance against the challenge tests. Conclusions: The results of this study investigated the positive effects of B. subtilis and B. circulance on Trichopodus trichopterus resistance time to the challenge test.