Abstract Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-Cas system
has been the focus since its first introduction to genome editing research. It acts as an
adaptive immune system in many prokaryotes by detecting and degrading viral/invasive
DNA/RNA repurposed for targeted genome editing in various organisms, including bacterial
species relevant to industrial biotechnology. Targeted genome editing in bacteria has been
implemented for multiple purposes, such as method development, understanding …