The aim of this study was to provide clinical results of dentinal desensitization treatment of non-carious defects. 146 patients were treated, who were diagnosed with generalized and local hyperesthesia of the second and third level. The patients were treated with Tiefenfluorid during 2 sessions carried out in interval of 8 days. Level of hyperesthesia and success of the treatment were assessed not only according to patient's complaints but also by using the test-meter for making evaluations in mkA. The opposite tooth side of the same patient, was treated with conventional fluoride gel to serve as control side. The patients were followed up for 2 years. The cases treated with Tiefenfluorid, which included treatment of general dental hyperesthesia, localized hyperesthesia in the tooth necks, as well as treatment of cuneiform defects, resulted more successful compared to the treatment with traditional fluoride gel. The immediate response as well as the long term result was better (it was necessary to perform 10 sessions of traditional fluoride gel treatment and only 2-3 treatments with Tiefenfluorid).