Structural determination of protein-protein complexes such as multidomain nuclear
receptors has been challenging for high-resolution structural techniques. Here, we present a
combined use of multiple biophysical methods, termed iSPOT, an integration of shape
information from small-angle X-ray scattering (SAXS), protection factors probed by hydroxyl
radical footprinting, and a large series of computationally docked conformations from rigid-
body or molecular dynamics (MD) simulations. Specifically tested on two model systems, the …