… We sought to identify and test a thermostable CRISPR-Cas9 … thermostable Cas9 enzymes
had not been reported and we therefore aimed to identify a previously uncharacterized Cas9 …

… for conferring the thermostability of ThermoCas9. First, … thermostability strongly depends on
the association with an appropriate sgRNA guide. This stabilization of the multi-domain Cas9 …

… as a thermostable Cas9 homolog and expand the temperatures at which Cas9 can be
used. We anticipate that the development of GeoCas9 will enhance the utility of CRISPR-Cas9 …

… Here, the cryo-electron microscopy structures of thermostable Cas9 from Geobacillus
stearothermophilus (GeoCas9) in a complex with sgRNA and target DNA are reported. The …

… In this study, we determined the structures of the thermostable TccCas13a protein with and
… pre-crRNA,36, 41 suggesting the thermostability of these two Cas13a proteins and the lack of …

… To address this issue, we developed a thermostable Cas9-based system for … a thermostable
Cas9-based engineering tool for gene editing in T. ethanolicus. The thermostable Cas9-…

CRISPR-Cas9 is a powerful technology that has rapidly transformed genome editing. The
Cas9 from Streptococcus pyogenes (SpCas9) is susceptible to degradation and unsuitable for …

… Currently, researchers are still exploring a pool of thermostable Cas9 nucleases, with novel
proteins being discovered and characterized. However, this subclass of Cas effectors has …

… nearly half of the known CRISPR–Cas9 family of enzymes, the Type II-C CRISPR–Cas9 has
been … Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease …

… Furthermore, we tried to evaluate whether the latest bacterial thermostable CRISPR/Cas9
system could be functionally developed in thermophilic fungi at the optimum growth …