Third-generation sequencing reveals extensive polycistronism and transcriptional overlapping in a baculovirus
The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is an insect-pathogen
baculovirus. In this study, we applied the Oxford Nanopore Technologies platform for the
analysis of the polyadenylated fraction of the viral transcriptome using both cDNA and direct
RNA sequencing methods. We identified and annotated altogether 132 novel transcripts and
transcript isoforms, including 4 coding and 4 non-coding RNA molecules, 47 length variants,
5 splice isoforms, as well as 23 polycistronic and 49 complex transcripts. All of the identified …
baculovirus. In this study, we applied the Oxford Nanopore Technologies platform for the
analysis of the polyadenylated fraction of the viral transcriptome using both cDNA and direct
RNA sequencing methods. We identified and annotated altogether 132 novel transcripts and
transcript isoforms, including 4 coding and 4 non-coding RNA molecules, 47 length variants,
5 splice isoforms, as well as 23 polycistronic and 49 complex transcripts. All of the identified …