Tissue handling and dissociation for single-cell RNA-seq
FA Vieira Braga, RJ Miragaia - Single Cell Methods: Sequencing and …, 2019 - Springer
Single Cell Methods: Sequencing and Proteomics, 2019•Springer
The starting material for all single-cell protocols is a cell suspension. The particular functions
and spatial distribution of immune cells generally make them easy to isolate them from the
tissues where they dwell. Here we describe tissue dissociation protocols that have been
used to obtain human immune cells from lymphoid and nonlymphoid tissues to be then used
as input to single-cell methods. We highlight the main factors that can influence the final
quality of single-cell data, namely the stress signatures that can bias its interpretation.
and spatial distribution of immune cells generally make them easy to isolate them from the
tissues where they dwell. Here we describe tissue dissociation protocols that have been
used to obtain human immune cells from lymphoid and nonlymphoid tissues to be then used
as input to single-cell methods. We highlight the main factors that can influence the final
quality of single-cell data, namely the stress signatures that can bias its interpretation.
Abstract
The starting material for all single-cell protocols is a cell suspension. The particular functions and spatial distribution of immune cells generally make them easy to isolate them from the tissues where they dwell. Here we describe tissue dissociation protocols that have been used to obtain human immune cells from lymphoid and nonlymphoid tissues to be then used as input to single-cell methods. We highlight the main factors that can influence the final quality of single-cell data, namely the stress signatures that can bias its interpretation.
Springer
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