We herein report the development of a molecular toolbox for the dimorphic fungus
Paracoccidioides brasiliensis, specifically a more efficient transformation and a gene
expression system. We evaluated several parameters that influence Agrobacterium
tumefaciens-mediated transformation (ATMT), such as co-cultivation conditions and host cell
susceptibility. Our results show that cellular recovery and air drying of A. tumefaciens: P.
brasiliensis mixtures are essential for ATMT. Overall, our data indicate a transformation …