Transcriptional profiling of reporter genes used for molecular imaging of embryonic stem cell transplantation
JC Wu, JM Spin, F Cao, S Lin, X Xie… - Physiological …, 2006 - journals.physiology.org
Stem cell therapy offers exciting promise for treatment of ischemic heart disease. Recent
advances in molecular imaging techniques now allow investigators to monitor cell fate
noninvasively and repetitively. Here we examine the effects of a triple-fusion reporter gene
on embryonic stem (ES) cell transcriptional profiles. Murine ES cells were stably transfected
with a self-inactivating lentiviral vector carrying a triple-fusion (TF) construct consisting of
fluorescence, bioluminescence, and positron emission tomography (PET) reporter genes …
advances in molecular imaging techniques now allow investigators to monitor cell fate
noninvasively and repetitively. Here we examine the effects of a triple-fusion reporter gene
on embryonic stem (ES) cell transcriptional profiles. Murine ES cells were stably transfected
with a self-inactivating lentiviral vector carrying a triple-fusion (TF) construct consisting of
fluorescence, bioluminescence, and positron emission tomography (PET) reporter genes …
[PDF][PDF] Transcriptional profiling of reporter genes used for
T Quertermous, Y Chen, AY Sheikh, RC Robbins… - Physiol …, 2006 - academia.edu
METHODS Culture of undifferentiated ES cells. The murine ES-D3 cell line (CRL-1934) was
obtained from the American Type Culture Collection (ATCC; Manassas, VA). Cells were
originally isolated from day 4 blastocytes of 129/Sv mice (12). ES cells were kept in an
undifferentiated, pluripotent state by using 1,000 IU/ml leukemia inhibitory factor (LIF;
Chemicon, ESGRO, ESG1107) and were grown on top of a murine embryonic fibroblast
feeder layer inactivated by 10 μg/ml mitomycin C (Sigma, St. Louis, MO). ES cells were …
obtained from the American Type Culture Collection (ATCC; Manassas, VA). Cells were
originally isolated from day 4 blastocytes of 129/Sv mice (12). ES cells were kept in an
undifferentiated, pluripotent state by using 1,000 IU/ml leukemia inhibitory factor (LIF;
Chemicon, ESGRO, ESG1107) and were grown on top of a murine embryonic fibroblast
feeder layer inactivated by 10 μg/ml mitomycin C (Sigma, St. Louis, MO). ES cells were …
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