Abstract Foot and Mouth Disease (FMD) is a very dangerous livestock disease which causes
a serious loss in the production of milk and meat. Therefore, producing an effective
recombinant subunit vaccine virus this disease is of great importance. Transient gene
expression is a valuable tool to reach rapid and acceptable recombinant vaccine. An
Agrobacterium-mediated transient gene expression assay was carried out in spinach
(Spinacia oleracea) leaves for expression of a chimeric gene encoding a part of capsid …

An Agrobacterium-mediated transient gene expression assay was carried out in alfalfa
(Medicago sativa) leaves for expression of a chimeric gene encoding a part of capsid protein
of Foot and Mouth Disease virus called VP1. The plant leaves were transformed via
agroinfiltration procedure. The presence of the foreign gene and its expression in
transformed plants were evaluated by polymerase chain reaction (PCR), real time PCR,
protein Dot blot and ELISA. Moreover, gene expression in the transformed leaves was …