A high-throughput optical biosensing technique is proposed and demonstrated. This hybrid
technique combines optical transmission of nanoholes with colorimetric silver staining. The
size and spacing of the nanoholes are chosen so that individual nanoholes can be
independently resolved in massive parallel using an ordinary transmission optical
microscope, and, in place of determining a spectral shift, the brightness of each nanohole is
recorded to greatly simplify the readout. Each nanohole then acts as an independent sensor …