… ABSTRACT We describe here a transposon-based DNA sequencing strategy that allows the
introduction of sequencing priming sites throughouta target sequence by bacterialmating. A …

… Then the properties of a special cloning vector for isolating transposon-facilitated nested …
Transposon-facilitated nested deletions show great promise for large-scale sequencing …

… strains, which we call "Tfr" (for Transposon-facilitated recombination) donors, are described.
… The term "Tfr" (Transposon-facilitated recombination) was chosen to denote our Hfr donors …

… them a sequence of DNA homologous to a DNA sequence present on the chromosome (14).
One approach has been to use recombinant DNA techniques to insert a chromosomal DNA …

… elements for large scale DNA sequencing: Our premise is that … DNA fragments, without the
inefficiencies of methods involving randon_ subcloning and attendant repetitive sequencing, …

… sequencing repetitive DNA are minimized. Bacterial transposons show great promise as tools
for both small and large-scale sequencing … to many locations in cloned DNA targets. Many …

… The use of transposons offers a parallel approach to the problem of sequencing a large … a
DNA sequence with the special property that it can move itself from one location in the genome …

… , and our results indicate that artificial transposon-mediated DNA sequencing is broadly
useful with 1- to 15-kb plasmid inserts cloned from a variety of sources (Table3). We have also …

… to ‘traditional’fluorescence-based sequencing. Four chapters present advantages and … for
large-scale genome sequencing: shotgun and transposon-facilitated directed sequencing. Liu …

… transposon-facilitated DNA sequencing strategy to be a highly cost-effective approach for
sequencing DNA … double-stranded elucidation of the sequences of cDNA clones. Furthermore, …