biological treatment of patients with gliomas. Glioma tissue was obtained from 16 patients,
tumor cell lines were established, and TILs were expanded in 16/16 cases using a
combination of IL-2/IL-15/IL-21. Intracellular cytokine staining (ICS, IL-2, IL-17, TNFα and
IFNγ production) as well as a cytotoxicity assay was used to detect TIL reactivity against
autologous tumor cells or shared tumor-associated antigens (TAAs; ie, NY-ESO-1, Survivin …