Tyrosinase immobilised on polyamide tubular membrane for the l-DOPA production: Total recycle and continuous reactor study
The production of l-3, 4-dihydroxyphenylalanine (l-DOPA) from l-tyrosine catalysed from
mushroom tyrosinase immobilised in a continuous membrane reactor was studied and
compared with free enzyme used in a stirred tank reactor. The enzyme was immobilised by
cross-flow filtration in asymmetric tubular membranes made of polyamide having nominal
molecular weight cut off of 20kDa. Results showed that the immobilization procedure did not
alter the catalytic properties of the enzyme. The immobilised tyrosinase showed high stability …
mushroom tyrosinase immobilised in a continuous membrane reactor was studied and
compared with free enzyme used in a stirred tank reactor. The enzyme was immobilised by
cross-flow filtration in asymmetric tubular membranes made of polyamide having nominal
molecular weight cut off of 20kDa. Results showed that the immobilization procedure did not
alter the catalytic properties of the enzyme. The immobilised tyrosinase showed high stability …
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