Protein ubiquitylation is an essential post-translational modification process that performs a
critical role in a wide range of biological functions, even a degenerative role in certain
diseases, and is consequently used as a promising target for the treatment of various
diseases. Owing to the significant role of protein ubiquitylation, these sites can be identified
by enzymatic approaches, mass spectrometry analysis, and combinations of
multidimensional liquid chromatography and tandem mass spectrometry. However, these …