Shampoo was a cosmetic preparation whose main function to clean the hair and scalp. Salicylic acid was a substance that was often used as the basis of addition to the product of dandruff shampoo treatment because its effect as keratolic can accelerate cell regeneration. Salicylic acid or salicylic acid levels were limited to 3.0% rinse according to BPOM. This study aims to determine the physical stability and salicylic acid levels contained in labeled (branded) shampoo preparations using the UV-Vis spectrophotometry method. The results showed a linear calibration curve with the regression equation y= 0.0092 x+ 0.1375 and the correlation coefficient (r) of 0.9997. The results showed that the validity test of UV-Vis spectrophotometry method has fulfilled the validation requirements. Three samples of anti-dandruff shampoo cosmetics were analyzed to contain salicylic acid. Salicylic acid levels in shampoo cosmetic products did not exceed the maximum and safe to use., each of them 0.0970%, 0.1796% and 0.2198%.