Ultrashort nucleic acid duplexes exhibit long wormlike chain behavior with force-dependent edge effects
KD Whitley, MJ Comstock, YR Chemla - Physical review letters, 2018 - APS
KD Whitley, MJ Comstock, YR Chemla
Physical review letters, 2018•APSDespite their importance in biology and use in nanotechnology, the elastic behavior of
nucleic acids on “ultrashort”(< 15 nt) length scales remains poorly understood. Here, we use
optical tweezers combined with fluorescence imaging to observe directly the hybridization of
oligonucleotides (7–12 nt) to a complementary strand under tension and to measure the
difference in end-to-end extension between the single-stranded and duplex states. Data are
consistent with long-polymer models at low forces (< 8 pN) but smaller than predicted at …
nucleic acids on “ultrashort”(< 15 nt) length scales remains poorly understood. Here, we use
optical tweezers combined with fluorescence imaging to observe directly the hybridization of
oligonucleotides (7–12 nt) to a complementary strand under tension and to measure the
difference in end-to-end extension between the single-stranded and duplex states. Data are
consistent with long-polymer models at low forces (< 8 pN) but smaller than predicted at …
Despite their importance in biology and use in nanotechnology, the elastic behavior of nucleic acids on “ultrashort” () length scales remains poorly understood. Here, we use optical tweezers combined with fluorescence imaging to observe directly the hybridization of oligonucleotides (7–12 nt) to a complementary strand under tension and to measure the difference in end-to-end extension between the single-stranded and duplex states. Data are consistent with long-polymer models at low forces () but smaller than predicted at higher forces (), the result of the sequence-dependent duplex edge effects.
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