This study focuses on the prospect of Jatropha Curcas seed residual from the ultrasonic in-situ process which is used as a biofuel raw material especially for producing bioethanol. Reactive extraction process coupled with ultrasonic system were used for simultaneous oil extraction and transesterification of Jatropha Curcas seed. Using ethanol as the solvent, alkaline catalyst (sodium hydroxide) and with the aid of ultrasonic device, about 50% oil from the initial seeds was extracted, which is equivalent to Soxhlet extraction performance. The seeds were being chemically and physically characterized with ultimate analyses, with SEM and XRD as potential bioethanol raw material. SEM and XRD profile exhibited loosen compounds in the ultrasonicated residues and provided a better accessible and easier degradable fiber for assisting bioethanol production process compared to the initial seeds. The positive effects of the ultrasonic reactive extraction for Jatropha Curcas seed pre-treatment is beneficial towards bioethanol production and could further be used as a solvent in the latter process.