Simple Summary Unraveling the mechanistic regulations that influence tumor behavior is an
important step towards treatment. However, in vitro studies capture only small parts of the
complex signaling cascades leading to tumor development. Mechanistic modeling, instead,
allows a more holistic view of complex signaling pathways and their crosstalk. These models
are able to suggest mechanistic regulations that can be validated by targeted and thus more
cost-effective experiments. This article presents a logical model of pancreatic cancer cells …

Cofilin-1 (CFL1) overexpression in pancreatic cancer correlates with high invasiveness and
shorter survival. Besides a well-documented role in actin remodeling, additional cellular
functions of CFL1 remain poorly understood. Here, we unraveled molecular tumor-
promoting functions of CFL1 in pancreatic cancer. For this purpose, we first show that a
knockdown of CFL1 results in reduced growth and proliferation rates in vitro and in vivo,
while apoptosis is not induced. By mechanistic modeling we were able to predict the …