Using high throughput sequencing to explore the biodiversity in oral bacterial communities
High throughput sequencing of 16S ribosomal RNA gene amplicons is a cost‐effective
method for characterization of oral bacterial communities. However, before undertaking
large‐scale studies, it is necessary to understand the technique‐associated limitations and
intrinsic variability of the oral ecosystem. In this work we evaluated bias in species
representation using an in vitro‐assembled mock community of oral bacteria. We then
characterized the bacterial communities in saliva and buccal mucosa of five healthy subjects …
method for characterization of oral bacterial communities. However, before undertaking
large‐scale studies, it is necessary to understand the technique‐associated limitations and
intrinsic variability of the oral ecosystem. In this work we evaluated bias in species
representation using an in vitro‐assembled mock community of oral bacteria. We then
characterized the bacterial communities in saliva and buccal mucosa of five healthy subjects …
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