Predicting the effect of a single amino acid substitution on the stability of a protein structure
is a fundamental task in macromolecular modeling. It has relevance to drug design and
understanding of disease-causing protein variants. We present KINARI-Mutagen, a web
server for performing in silico mutation experiments on protein structures from the Protein
Data Bank. Our rigidity-theoretical approach permits fast evaluation of the effects of
mutations that may not be easy to perform in vitro, because it is not always possible to …

Predicting how a single amino acid substitution affects the stability of a protein structure is a
fundamental task in macromolecular modeling. It has relevance to drug design and
understanding of disease-causing protein variants. We present KINARI-Mutagen, a web
server for performing in silico mutation experiments on protein structures from the Protein
Data Bank. Our rigidity-based approach permits fast evaluation of the effects of mutations
that may not be easy to perform in vitro, because it is not always possible to express a …