The aim of the present study was to examine the validity of Prosocial Tendency Measure (PTM) in Iranian students. This scale measures 6 types of prosocial tendencies including altruistic, anonymous, dire, emotional, compliant and public prosocial tendencies (Carlo and Randal, 2002). We used 182 undergraduate students in order to study the validity of the scale by confirmatory factor analysis method, internal consistency and relationships to other measures. Also 34 students were used for test retest reliability procedure. We studied the relationships of the measure s’ subscales to additional items, empathy, religiosity and social desirability. The results showed that this measure has acceptable fitness on Carlo and Randall (2002) model. Also the subscales of this measure had acceptable internal consistency and reliability after test retest. The subscales of compliant, emotional, anonymous and altruism had positive and significant relationships with empathy but there was negative relationship between public to empathy. The subscales of compliant and anonymous had positive and significant relationships with religiosity. Social desirability had negative and significant correlation to public. But social desirability had also positive and significant relationships to anonymous and altruism. There was not any significant difference between girls and boys in any of six PTM subscales.