Validation of reference genes for quantitative real-time PCR during leaf and flower development in Petunia hybrida
Background Identification of genes with invariant levels of gene expression is a prerequisite
for validating transcriptomic changes accompanying development. Ideally expression of
these genes should be independent of the morphogenetic process or environmental
condition tested as well as the methods used for RNA purification and analysis. Results In
an effort to identify endogenous genes meeting these criteria nine reference genes (RG)
were tested in two Petunia lines (Mitchell and V30). Growth conditions differed in Mitchell …
for validating transcriptomic changes accompanying development. Ideally expression of
these genes should be independent of the morphogenetic process or environmental
condition tested as well as the methods used for RNA purification and analysis. Results In
an effort to identify endogenous genes meeting these criteria nine reference genes (RG)
were tested in two Petunia lines (Mitchell and V30). Growth conditions differed in Mitchell …
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