Survey was conducted in Uttar Pradesh (Lucknow, Varanasi and adjoining areas) and Jharkhand (Ranchi and adjoining areas) to find out the existing natural variability among the jamun seedling trees and to identify superior genotypes with good fruit qualities. Observations on the physico-chemical parameters of fruits were recorded on 32 genotypes. All the genotypes showed considerable variability with respect to the physico-chemical characters assessed. The genotypes, RNC-26 and RNC-11 were found promising and had higher fruit and pulp weight with sweet fruits. Highest pulp content (97.71%) was recorded in V-8 followed by V-6 (95.84 %) and V-7 (93.81%) genotypes collected from Varanasi region. Thin seed with almost negligible seed weight (0.12 g) was observed in V-8 followed by V-6 (0.16 g) and V-7 (0.31 g). Hence, these genotypes might be used as seedless jamun.