Very fast CRISPR on demand

Y Liu, R Zou, Y Nihongaki, S He, S Razavi, B Wu… - Biophysical Journal, 2020 - cell.com
CRISPR/Cas systems have enormous potential as tools for genome editing and for studying
DNA damage response through convenient RNA-guided genomic targeting. We developed …

[HTML][HTML] Glen Report 32–24: Very Fast CRISPR (vfCRISPR)“On Demand

G Zon, T Biotechnologies - glenresearch.com
… In modern molecular biology and biotechnology, very few methods have been met with as
much success as CRISPR, as evidenced by this chart of publications indexed to CRISPR in …

Fast and sensitive CRISPR detection by minimized interference of target amplification

X Tong, K Zhang, Y Han, T Li, M Duan, R Ji… - Nature Chemical …, 2024 - nature.com
Despite the great potential of CRISPR-based detection, it has not been competitive with other
market diagnostics for on-site and in-home testing. Here we dissect the rate-limiting factors …

[引用][C] Very fast CRISPR on demand

S Mao - Science, 2020 - ui.adsabs.harvard.edu
Very fast CRISPR on demand - NASA/ADS … Very fast CRISPR on demand

Amplification-free CRISPR/Cas detection technology: challenges, strategies, and perspectives

H Li, Y Xie, F Chen, H Bai, L Xiu, X Zhou… - Chemical Society …, 2023 - pubs.rsc.org
… of COVID-19, such a fast turnaround time of amplification-free CRISPR/Cas-based detection
… portable signal collectors in digital-CRISPR detection assays, to meet the market demand. …

Recent developments and future directions in point-of-care next-generation CRISPR-based rapid diagnosis

YM Hassan, AS Mohamed, YM Hassan… - Clinical and …, 2025 - Springer
… The demand for sensitive, rapid, and affordable diagnostic techniques has surged, …
focus in CRISPR diagnostic innovations. This review examines the current state of CRISPR-based …

“Off-Spotter”: very fast and exhaustive enumeration of genomic lookalikes for designing CRISPR/Cas guide RNAs

V Pliatsika, I Rigoutsos - Biology direct, 2015 - Springer
… It appears to the reviewer that the area is evolving fast due to general demand and there
is quite some literature out already. Unfortunately, the authors do not elaborate much about …

In vitro cleavage and electrophoretic mobility shift assays for very fast crispr

RS Zou, Y Liu, T Ha - Bio-protocol, 2021 - bio-protocol.org
… [Abstract] CRISPR-Cas9 has transformed biomedical … through the recently developed very
fast CRISPR (vfCRISPR) … Heating at 95C rapidly inactivates the RNP on demand, allowing …

Photocontrolled crRNA activation enables robust CRISPR-Cas12a diagnostics

M Hu, Z Qiu, Z Bi, T Tian, Y Jiang… - Proceedings of the …, 2022 - National Acad Sciences
… , which are in demand almost everywhere in … very fast (usually a few seconds) and contactless
manner. This strategy has been widely employed to spatiotemporally regulate the CRISPR

Mapping cellular responses to DNA double-strand breaks using CRISPR technologies

Y Liu, WT Cottle, T Ha - Trends in Genetics, 2023 - cell.com
… (CRISPR)–CRISPR-associated protein 9 (Cas9) has reinvigorated interest in DSB repair
mechanisms. This review surveys the latest methodological advances and biological insights …