Interest in spatial omics is on the rise, but generation of highly multiplexed images remains
challenging, due to cost, expertise, methodical constraints, and access to technology. An
alternative approach is to register collections of whole slide images (WSI), generating
spatially aligned datasets. WSI registration is a two-part problem, the first being the
alignment itself and the second the application of transformations to huge multi-gigapixel
images. To address both challenges, we developed Virtual Alignment of pathoLogy Image …

Interest in spatial omics is on the rise, but generation of highly multiplexed images used in
many spatial analyses remains challenging, due to cost, expertise, methodical constraints,
and/or access to technology. An alternative to performing highly multiplexed staining is to
register collections of whole slide images (WSI), creating a collection of aligned images that
can undergo spatial analyses. However, registration of WSI is two part problem, with the first
being the alignment itself, and the second being the application of the transformations to …