[PDF][PDF] development and validation of a RP-HPLC method for simultaneous estimation of lamivudine, tenofovir disoproxil fumarate and efavirenz in a combined tablet …
P Vanaja, N Anusha, VS Prasad - Int J Pharm Pharm Sci, 2013 - scholar.archive.org
P Vanaja, N Anusha, VS Prasad
Int J Pharm Pharm Sci, 2013•scholar.archive.orgObjective: Development of an accurate, simple, precise and rapid method for estimating
Tenofovir disoproxil fumarate, Lamivudine and Efavirenz simultaneously, in a combined
tablet form. Methods: The method uses Reverse Phase High Performance Liquid
Chromatography (RP-HPLC). Symmetry C18 (4.6 x 100 mm, 3.5 µm) column operated with a
mixture of phosphate buffer of pH 4.0 with ortho phosphoric acid and Acetonitrile (42: 58) as
mobile phase was found to be suitable for the simultaneous estimation. The flow rate was …
Tenofovir disoproxil fumarate, Lamivudine and Efavirenz simultaneously, in a combined
tablet form. Methods: The method uses Reverse Phase High Performance Liquid
Chromatography (RP-HPLC). Symmetry C18 (4.6 x 100 mm, 3.5 µm) column operated with a
mixture of phosphate buffer of pH 4.0 with ortho phosphoric acid and Acetonitrile (42: 58) as
mobile phase was found to be suitable for the simultaneous estimation. The flow rate was …
Abstract
Objective: Development of an accurate, simple, precise and rapid method for estimating Tenofovir disoproxil fumarate, Lamivudine and Efavirenz simultaneously, in a combined tablet form.
Methods: The method uses Reverse Phase High Performance Liquid Chromatography (RP-HPLC). Symmetry C18 (4.6 x 100 mm, 3.5 µm) column operated with a mixture of phosphate buffer of pH 4.0 with ortho phosphoric acid and Acetonitrile (42: 58) as mobile phase was found to be suitable for the simultaneous estimation. The flow rate was maintained at 0.5 mL/min. Detection was carried out at 254 nm using a UV detector.
Results: The total run time was less than 11 minutes with the retention times of 2.2 min, 3.3 min and 10.8 min respectively for Lamivudine, Tenofovir disoproxil fumarate and Efavirenz.
Conclusion: Validation of the method was performed for precision, accuracy, linearity, ruggedness, specificity and sensitivity to conform to the ICH guidelines for validation of an analytical method.
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