Applying CRISPR/Cas system as a signal enhancer for DNAzyme-based lead ion detection

Y Chen, H Wu, S Qian, X Yu, H Chen, J Wu - Analytica Chimica Acta, 2022 - Elsevier
Heavy metal lead accumulation in the environment pollutes the ecology systems and further
threatens the human health. It is necessary to develop a sensitive method to detect it. Here …

An ultrasensitive Cd2+ detection biosensor based on DNAzyme and CRISPR/Cas12a coupled with hybridization chain reaction

P Liang, B Lv, K Chen, W Qiao, D Li - Analytica Chimica Acta, 2023 - Elsevier
The detection of cadmium is essential because it poses a significant threat to human health
and the environment. Recent advancements in biosensors that detect nonnucleic-acid …

A triple amplification strategy using GR-5 DNAzyme as a signal medium for ultrasensitive detection of trace Pb2+ based on CRISPR/Cas12a empowered …

Y Yue, S Wang, Q Jin, N An, L Wu, H Huang - Analytica Chimica Acta, 2023 - Elsevier
Abstract Lead ions (Pb 2+) are a well-known toxic heavy metal that poses a significant threat
to human health. Therefore, the development of a simple and ultrasensitive technique for …

Nanozyme-catalysed CRISPR-Cas12a system for the preamplification-free colorimetric detection of lead ion

S Xu, S Wang, L Guo, Y Tong, L Wu, X Huang - Analytica Chimica Acta, 2023 - Elsevier
CRISPR-based detection was often based on the target preamplification to realize the high
sensitivity. Here, we prepared a CRISPR-Cas12a system for the colorimetric detection of …

Dual-functional DNAzyme powered CRISPR-Cas12a sensor for ultrasensitive and high-throughput detection of Pb2+ in freshwater

J Wen, H Deng, D He, Y Yuan - Science of The Total Environment, 2024 - Elsevier
Freshwater lead pollution has posed severe threat to the environment and human health,
underscoring the urgent necessity for accurate and user-friendly detection methods. Herein …

Csm6-DNAzyme tandem assay for one-pot and sensitive analysis of lead pollution and bioaccumulation in mice

H Yang, F Li, T Xue, MR Khan, X Xia… - Analytical …, 2022 - ACS Publications
Lead contamination in the environment tends to enter the food chain and further into the
human body, causing serious health issues. Herein, we proposed a Csm6-DNAzyme …

CRISPR/Cas12a-derived ratiometric fluorescence sensor for high-sensitive Pb2+ detection based on CDs@ ZIF-8 and DNAzyme

C Yang, C Du, F Yuan, P Yu, B Wang, C Su… - Biosensors and …, 2024 - Elsevier
Benefiting from specific target recognition and trans-cleavage capabilities, the
CRISPR/Cas12a system has great application prospects in the design of highly sensitive …

DNA nanocage confined DNAzyme for detection of lead ions coupled with CRISPR-Cas12a system

Y Yu, Y Zhang, W Li, Z Wang, J Zhang - Chemical Engineering Journal, 2024 - Elsevier
As one of the most toxic heavy metals, trace lead ions (Pb 2+) can cause serious harm to
human, so rapid detection of trace Pb 2+ has always attracted wide attention. DNAzyme is …

CRISPR/Cas Systems: Endless Possibilities for Electrochemical Nucleic Acid Sensors

X Zhuang, X Yang, B Cao, H Sun, X Lv… - Journal of The …, 2022 - iopscience.iop.org
The CRISPR/Cas system has gained enormous attention for its excellent gene-editing
capabilities. In recent years, the reported trans-cleavage activity of some Cas proteins …

DNAzyme-based cascade signal amplification strategy for highly sensitive detection of lead ions in the environment

J Xu, M Liu, W Zhao, S Wang, M Gui, H Li… - Journal of Hazardous …, 2022 - Elsevier
Lead ions are one of many common environmental pollutants, that can cause posing a
serious threat to people's health, thus, their efficient and sensitive detection is important. We …