tRNA-guanine transglycosylase from Escherichia coli: gross tRNA structural requirements for recognition

AW Curnow, FL Kung, KA Koch, GA Garcia - Biochemistry, 1993 - ACS Publications
Revised Manuscript Received February 25, 1993 abstract: tRNA-guanine transglycosylase
(TGT) is the enzyme responsible for the post-transcriptional modification of specific tRNAs …

tRNA-guanine transglycosylase from Escherichia coli: overexpression, purification and quaternary structure

GA Garcia, KA Koch, S Chong - Journal of molecular biology, 1993 - Elsevier
Abstract tRNA-guanine transglycosylase (TGT) is the enzyme responsible for the post-
transcriptional modification of specific tRNAs (Asn, Asp, His and Tyr) with queuine. In E. coli …

tRNA-guanine transglycosylase from Escherichia coli: Minimal tRNA structure and sequence requirements for recognition

AW Curnow, GA Garcia - Journal of Biological Chemistry, 1995 - ASBMB
Previously, we have demonstrated that the tRNA-guanine transglycosylase (TGT) from
Escherichia coli is capable of utilizing an in vitro generated minihelix consisting of the …

tRNA-guanine transglycosylase from Escherichia coli: Recognition of dimeric, unmodified tRNATyr

AW Curnow, GA Garcia - Biochimie, 1994 - Elsevier
In order to probe the interaction between tRNA and the tRNA hypermodifying enzyme, tRNA-
guanine transglycosylase (TGT) from Escherichia coli, we have undertaken the generation …

tRNA–guanine transglycosylase from Escherichia coli: Recognition of noncognate–cognate chimeric tRNA and discovery of a novel recognition site within the TΨC …

FL Kung, S NONEKOWSKI, GA GARCIA - Rna, 2000 - cambridge.org
tRNA–guanine transglycosylase (TGT) is a key enzyme involved in the posttranscriptional
modification of tRNA across the three kingdoms of life. In eukaryotes and eubacteria, TGT is …

tRNA−Guanine Transglycosylase from Escherichia coli:  Molecular Mechanism and Role of Aspartate 89

JD Kittendorf, LM Barcomb, ST Nonekowski… - Biochemistry, 2001 - ACS Publications
The enzyme tRNA− guanine transglycosylase (TGT, EC 2.4. 2.29) catalyzes a
posttranscriptional transglycosylation reaction involved in the incorporation of the modified …

The Escherichia coli tRNA-guanine transglycosylase can recognize and modify DNA

ST Nonekowski, FL Kung, GA Garcia - Journal of Biological Chemistry, 2002 - ASBMB
tRNA-guanine transglycosylase (TGT) catalyzes the exchange of queuine (or a precursor)
for guanine 34 in tRNA. The minimal RNA recognition motif for TGT has been found to …

tRNA-guanine transglycosylase from Escherichia coli: structure-activity studies investigating the role of the aminomethyl substituent of the heterocyclic substrate PreQ1

GC Hoops, LB Townsend, GA Garcia - Biochemistry, 1995 - ACS Publications
Revised Manuscript Received August 14, 1995® abstract: A series of 5-substituted 2-
aminopyrrolo [2, 3-< i] pyrimidin-4 (3//)-ones have been synthesized in order to study the …

A UGU sequence in the anticodon loop is a minimum requirement for recognition by Escherichia coli tRNA-guanine transglycosylase.

S Nakanishi, T Ueda, H Hori, N Yamazaki… - Journal of Biological …, 1994 - Elsevier
Escherichia coli tRNA-guanine transglycosylase is an enzyme which catalyzes replacement
of guanine (G34) of tRNA (Asp), tRNA (Asn), tRNA (His) and tRNA (Tyr) by free guanine or …

An essential role for aspartate 264 in catalysis by tRNA-guanine transglycosylase from Escherichia coli

JD Kittendorf, T Sgraja, K Reuter, G Klebe… - Journal of Biological …, 2003 - ASBMB
tRNA-guanine transglycosylase (TGT) catalyzes a post-transcriptional base-exchange
reaction involved in the incorporation of the modified base queuine (Q) into the wobble …