A key element of successful lipidomics analysis is a sufficient extraction of lipid molecules
typically by two-phase systems such as chloroform-based Bligh and Dyer (B&D). However …

The goal of this research was to find the most comprehensive lipid extraction of blood
plasma, while also providing adequate aqueous preparation for metabolite analysis …

In order to profile the lipidome for untargeted lipidomics applications, analysis by ultra-high
performance liquid chromatography–high resolution mass spectrometry (UHPLC-HRMS) …

Lipidomics is a rapidly developing field in modern biomedical research. While LC-MS
systems are able to detect most of the known lipid classes in a biological matrix, there is no …

An unbiased sample preparation free of interferents (ie, competing analytes, detergents,
plastics) is critical to any lipid MS workflow. Here we present a novel three-phase lipid …

Lipid extraction is a critical step in sample preparation of lipidomics studies. Biphasic liquid-
liquid extraction protocol with methyl tert-butyl ether (MTBE)/methanol (MeOH) as organic …

Exploratory or untargeted ultra performance liquid chromatography–mass spectrometry
(UPLC–MS) profiling offers an overview of the complex lipid species diversity present in …

Lipidomic approaches are now widely used to investigate the relationship between lipid
metabolism, health and disease. Large-scale lipidomics studies typically aim to quantify …

Over the last decade, technological advances have improved the sensitivity and selectivity of
LC/MS analyzers, providing very efficient tools for lipidomics research. In particular, the nine …

In recent years, high-throughput lipid profiling has contributed to understand the biological,
physiological and pathological roles of lipids in living organisms. Across all kingdoms of life …