Publisher Summary The chapter focuses on the application of different ultrastructural
cytochemical methods that contributed an important way to the understanding of the …

In this review we describe major contributions of light and electron microscopic approaches
to the present understanding of functional nuclear architecture. The large gap of knowledge …

Publisher Summary This chapter provides an overview to both standard and advanced
methods for the sample preparation, staining, and structural analysis of nuclear architecture …

The study of the ultrastructural organization of the nuclear components experienced a great
acceleration during the 1960s with the introduction into electron microscopy of epoxy resins …

One of the ultimate goals of biological research is to understand mechanisms of cell function
within living organisms. With this in mind, many sophisticated technologies that allow us to …

For many years, ultrastructural cytochemistry has been a major tool for investigating the
relationships between the structure and the functions of the cell nucleus. This article shortly …

We investigated the nuclear ultrastructure of HeLa cells using cryotechniques. The cells
were cultured as monolayers, quick‐frozen and cryosubstituted in acetone without chemical …

Thie report illustrates intranuclear inclusions in living cells by phase-contrast and time-lapse
cinemicroecopy. As stated in reports by electron microscopists, the intranuclear inclusions …

Immobilized proteins within the nucleus are usually identified by treating cells with
detergent. The detergent-resistant fraction is often assumed to be chromatin and is …

Ultrastructural cytochemistry has been, for many years now, a major tool for investigating
structure–function relationships in the cell nucleus. It has been essential in approaching the …