tRNA−Guanine Transglycosylase from Escherichia coli:  Molecular Mechanism and Role of Aspartate 89

JD Kittendorf, LM Barcomb, ST Nonekowski… - Biochemistry, 2001 - ACS Publications
The enzyme tRNA− guanine transglycosylase (TGT, EC 2.4. 2.29) catalyzes a
posttranscriptional transglycosylation reaction involved in the incorporation of the modified …

An essential role for aspartate 264 in catalysis by tRNA-guanine transglycosylase from Escherichia coli

JD Kittendorf, T Sgraja, K Reuter, G Klebe… - Journal of Biological …, 2003 - ASBMB
tRNA-guanine transglycosylase (TGT) catalyzes a post-transcriptional base-exchange
reaction involved in the incorporation of the modified base queuine (Q) into the wobble …

tRNA-guanine transglycosylase from Escherichia coli: overexpression, purification and quaternary structure

GA Garcia, KA Koch, S Chong - Journal of molecular biology, 1993 - Elsevier
Abstract tRNA-guanine transglycosylase (TGT) is the enzyme responsible for the post-
transcriptional modification of specific tRNAs (Asn, Asp, His and Tyr) with queuine. In E. coli …

tRNA-guanine transglycosylase from Escherichia coli: gross tRNA structural requirements for recognition

AW Curnow, FL Kung, KA Koch, GA Garcia - Biochemistry, 1993 - ACS Publications
Revised Manuscript Received February 25, 1993 abstract: tRNA-guanine transglycosylase
(TGT) is the enzyme responsible for the post-transcriptional modification of specific tRNAs …

Mutagenesis and Crystallographic Studies of Zymomonas mobilis tRNA-Guanine Transglycosylase Reveal Aspartate 102 as the Active Site Nucleophile,

C Romier, K Reuter, D Suck, R Ficner - Biochemistry, 1996 - ACS Publications
Procaryotic tRNA-guanine transglycosylase (TGT) catalyzes the posttranscriptional base
exchange of the queuine precursor 7-aminomethyl-7-deazaguanine (preQ1) with the …

Identification of the Rate-Determining Step of tRNA-Guanine Transglycosylase from Escherichia coli

GA Garcia, SM Chervin, JD Kittendorf - Biochemistry, 2009 - ACS Publications
The modified RNA base queuine [7-(4, 5-cis-dihydroxy-1-cyclopenten-3-ylaminomethyl)-7-
deazaguanine] is present in tRNA because of a unique base-exchange process catalyzed …

tRNA–guanine transglycosylase from E. coli: a ping-pong kinetic mechanism is consistent with nucleophilic catalysis

DAM Goodenough-Lashua, GA Garcia - Bioorganic chemistry, 2003 - Elsevier
tRNA–guanine transglycosylase (TGT) is a key enzyme in the post-transcriptional
modification of certain tRNAs with the pyrrolopyrimidine base queuine. TGT is required for …

tRNA-guanine transglycosylase from Escherichia coli: Minimal tRNA structure and sequence requirements for recognition

AW Curnow, GA Garcia - Journal of Biological Chemistry, 1995 - ASBMB
Previously, we have demonstrated that the tRNA-guanine transglycosylase (TGT) from
Escherichia coli is capable of utilizing an in vitro generated minihelix consisting of the …

tRNA-guanine transglycosylase from Escherichia coli: Recognition of dimeric, unmodified tRNATyr

AW Curnow, GA Garcia - Biochimie, 1994 - Elsevier
In order to probe the interaction between tRNA and the tRNA hypermodifying enzyme, tRNA-
guanine transglycosylase (TGT) from Escherichia coli, we have undertaken the generation …

tRNA–guanine transglycosylase from Escherichia coli: Recognition of noncognate–cognate chimeric tRNA and discovery of a novel recognition site within the TΨC …

FL Kung, S NONEKOWSKI, GA GARCIA - Rna, 2000 - cambridge.org
tRNA–guanine transglycosylase (TGT) is a key enzyme involved in the posttranscriptional
modification of tRNA across the three kingdoms of life. In eukaryotes and eubacteria, TGT is …