The presence and folding pattern of chromatin in eukaryotic cells remain elusive and
controversial. In this study, we prepared ultra-thin sections of Hela cells with three different …

Highlights•Cryo-electro microscopy reveals the native conformation of chromatin fiber both
in vitro and in situ.•Presence of linker histone and ionic conditions determines the level of …

Although the formation of 30-nm chromatin fibers is thought to be the most basic event of
chromatin compaction, it remains controversial because high-resolution imaging of …

The naked DNA inside the nucleus interacts with proteins and RNAs forming a higher order
chromatin structure to spatially and temporally control transcription in eukaryotic cells. The …

Studies of chromatin extensibility have revealed the flow of chromatin and DNA from cell
nuclei and chromosomes in response to gravity or mechanical stretch following lysis by …

Since Flemming described a nuclear substance in the nineteenth century and named it
“chromatin,” this substance has fascinated biologists. What is the structure of chromatin …

A long strand of DNA is wrapped around the core histone and forms a nucleosome.
Although the nucleosome has long been assumed to be folded into 30-nm chromatin fibres …

The use of imaging techniques has become an essential tool in cell biology. In particular,
advances in fluorescence microscopy and conventional transmission electron microscopy …

Non-destructive electron microscopy of native chromatin from rat liver nuclei reveals that the
30 nm fibre is formed of four 11 nm nucleofilaments, arranged in a coiled-coil (or rope-like) …

The folding of the chromatin filament and, in particular, the organization of genomic DNA
within metaphase chromosomes has attracted the interest of many laboratories during the …