After gradually moving away from preparation methods prone to artefacts such as plastic
embedding and negative staining for cell sections and single particles, the field of cryo …

Cryo-electron microscopy (cryo-EM) is a structural molecular and cellular biology technique
that has experienced major advances in recent years. Technological developments in image …

Transmission electron microscopy (EM) is a versatile technique that can be used to image
biological specimens ranging from intact eukaryotic cells to individual proteins> 150 kDa …

Over the last 5 years what has been called the “Resolution Revolution”(Kühlbrandt, 2014) in
cryo-electron microscopy (cryo-EM) has opened a new era in structural biology. It is now …

Classical structural biology approaches rely on highly purified molecules which are isolated
from their neighbors far from the complex macromolecular interaction network of the cell (ex …

There has been enormous progress during the last few years in the determination of three-
dimensional biological structures by single particle electron cryomicroscopy (cryoEM) …

Cryo‐electron microscopy (cryo‐EM) is increasingly becoming a mainstream technology for
studying the architecture of cells, viruses and protein assemblies at molecular resolution …

All steps of cryogenic electron-microscopy (cryo-EM) workflows have rapidly evolved over
the last decade. Advances in both single-particle analysis (SPA) cryo-EM and cryo-electron …

Cryo-electron microscopy (cryo-EM) has become the technique of choice for structural
biology of macromolecular assemblies, after the 'resolution revolution'that has occurred in …

Single particle cryo-EM has recently developed into a powerful tool to determine the 3D
structure of macromolecular complexes at near-atomic resolution, which allows structural …