Contrast in electron cryo-microscopy (cryo-EM) is limited by the weak phase and radiation
sensitive nature of biologic samples embedded in vitrified ice. We have recently shown that …

The Volta phase plate is a recently developed electron cryo-microscopy (cryo-EM) device
that enables contrast enhancement of biological samples. Here we have evaluated the …

Cryo-electron microscopy (cryo-EM) is a powerful tool for macromolecular to near-atomic
resolution structure determination in the biological sciences. The specimen is maintained in …

Publisher Summary This chapter describes the cryoelectron microscopic analysis of
nucleosomes and chromatin. Cryoelectron microscopy is the technique of choice when …

The use of a Zernike-type phase plate in biologic cryo-electron microscopy allows the
imaging, without using defocus, of what are predominantly phase objects. It is thought that …

After gradually moving away from preparation methods prone to artefacts such as plastic
embedding and negative staining for cell sections and single particles, the field of cryo …

Highlights•Phase plates are an emerging technology in cryo-electron microscopy (cryo-
EM).•In cryo-electron tomography (cryo-ET) phase plates allow the visualization of cellular …

We investigate potential improvements in using electron cryomicrosopy (cryoEM) to image
thick specimens with high-resolution phase contrast imaging. In particular, using model …

Macromolecular imaging and tomography of ice embedded samples has developed into a
mature imaging technology, in structural biology today widely referred to simply as cryo …

Single particle cryo-electron microscopy (cryoEM) is becoming widely adopted as a tool for
structural characterization of biomolecules at near-atomic resolution. Vitrification of the …