Aurora M. Land el, Yosef, Michael A. Raftery,* and Giuseppe Attardi abstract: The possibility
of fractionating HeLa cell metaphase chromosomes by the electrofocusing technique has …

Isolated metaphase chromosomes from HeLa cells, mouse L cells, and Syrian hamster
embryonic fibroblast cells have been fractionated on the basis of size by linear sucrose …

Publisher Summary The nucleochromatin material is extracted from interphase cells;
isolated metaphase chromosomes offer unique possibilities for studying the genetic material …

Chromosome preparations can principally be obtained from all tissues and suspensions in
which mitoses occur. The initiation of cell cultures particularly suitable for chromosomal …

TECHNIQUES for the isolation of interphase chromosomes from nuclei of vertebrate tissues
were described in 1947 by Mirsky and Ris [19]. The determination of the chemical …

Modern cytological and culture techniques have made metaphase chromosomes of
mammalian cells among the most readily available for study; only 10 years ago they were …

A method for the isolation of metaphase chromosomes from mouse L1210 leukemia cells
has been developed. Cells, arrested at metaphase with colchicine, were exposed to …

Publisher Summary This chapter discusses the analytical techniques for isolated metaphase
chromosome fractions. Metaphase chromosomes represent reproducible precise …

Chinese hamster cells in culture were synchronized, collected at metaphase, homogenized
to release the chromosomes, and the chromosomes fractionated in a sucrose gradient using …

Materials and methods One of the major difficulties in the study of metaphase chromosome
structure is the lack of an easy way to obtain large numbers of dividing cells. This can be …