Electron microscopic and biochemical studies demonstrate that the fundamental structure of
chromatin depleted of lysine-rich histones is composed of a flexible chain of spherical …

We describe the results of a systematic study, using electron microscopy, of the effects of
ionic strength on the morphology of chromatin and of H1-depleted chromatin. With …

Robert T. Simpson abstract: I have prepared chromatin particles from chicken erythrocytes
which contain a 160 base pair length of DNA, an octamer of the four smaller histones, and a …

Histone-depleted nuclei were prepared from, isolated HeLa nuclei by extracting the histones
and other proteins with polyanions (dextran sulphate and heparin) or with high salt …

Chromatin prepared by brief digestion of nuclei with micrococcal nuclease, and extracted in
0.2 mM EDTA, appears in the electron microscope as filaments of about 100 A diameter …

Summary Chromatin subunits (“nucleosomes”) which were purified by sucrose gradient
centrifugation of a staphylococcal nuclease digest of chromatic, have been studied. We …

There is considerable current interest in the organisation of nucleosomes in chromatin. A
strong X-ray and neutron semi-meridional diffraction peak at approximately 10 nm had …

A high-resolution map for the arrangement of histones along DNA in the nucleosome core
particle has been determined by a sequencing procedure based on crosslinking histones to …

Both intact and nuclease-isolated chromatin fibers have been examined at different degrees
of salt-induced compaction, using a variety of preparation techniques. The results suggest …

We have found that DNAase I digestion of yeast, HeLa and chicken erythrocyte nuclei
produces a pattern of DNA fragments spaced 10 bases apart and extending to at least 300 …