CRISPR-Cas9 is a powerful technology that has rapidly transformed genome editing. The
Cas9 from Streptococcus pyogenes (SpCas9) is susceptible to degradation and unsuitable …

CRISPR-Cas9 is a widely used biochemical tool with applications in molecular biology and
precision medicine. The RNA-guided Cas9 protein uses its HNH endonuclease domain to …

The Streptococcus pyogenes Cas9 (SpCas9) protein is the centerpiece of a transformative
gene editing technology, widely used in molecular biology and precision medicine …

CRISPR–Cas9 is a widely employed genome-editing tool with functionality reliant on the
ability of the Cas9 endonuclease to introduce site-specific breaks in double-stranded DNA …

Allosteric signaling within multidomain proteins is a driver of communication between
spatially distant functional sites. Understanding the mechanism of allosteric coupling in large …

The RNA-guided CRISPR-Cas9 nuclease from Streptococcus pyogenes (SpCas9) has been
widely repurposed for genome editing. DNA cleavage of Cas9 is controlled by the …

At the core of the CRISPR–Cas9 genome-editing technology, the endonuclease Cas9
introduces site-specific breaks in DNA. However, precise mechanistic information to …

HNH is one of two endonuclease domains of the clustered regularly interspaced short
palindromic repeats (CRISPR)-associated protein Cas9 that perform site-specific cleavage …

The Cas9 endonuclease is widely used for genome engineering applications by
programming its single-guide RNA, and ongoing work is aimed at improving the accuracy …

CRISPR-Cas9 technology has been widely used for genome engineering. Its RNA-guided
endonuclease Cas9 binds specifically to target DNA and then cleaves the two DNA strands …