[HTML][HTML] TRIzol-based RNA extraction for detection protocol for SARS-CoV-2 of coronavirus disease 2019

A Amirouche, D Ait-Ali, H Nouri… - New microbes and new …, 2021 - Elsevier
Diagnostic testing is important for managing the 2019 novel coronavirus (SARS-CoV-2). We
developed an optimized protocol for SARS-CoV-2 RNA extraction from the surface of the …

[HTML][HTML] SARS-CoV-2: Comparative analysis of different RNA extraction methods

C Ambrosi, C Prezioso, P Checconi, D Scribano… - Journal of virological …, 2021 - Elsevier
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the etiologic agent of the
COVID-19 pandemic. Although other diagnostic methods have been introduced, detection of …

Alternative RNA extraction-free techniques for the real-time RT-PCR detection of SARS-CoV-2 in nasopharyngeal swab and sputum samples

SD Villota, VE Nipaz, A Carrazco-Montalvo… - Journal of virological …, 2021 - Elsevier
Standard diagnoses of SARS-CoV-2 infections are done by RNA extraction and real-time RT-
PCR (rRT-PCR). However, the need for RNA extraction complicates testing due to increased …

Automated SARS-COV-2 RNA extraction from patient nasopharyngeal samples using a modified DNA extraction kit for high throughput testing

H Al-Saud, K Al-Romaih, R Bakheet… - Annals of Saudi …, 2020 - annsaudimed.net
ABSTRACT BACKGROUND: The pandemic of severe acute respiratory syndrome
coronavirus 2 (SARS-COV-2) has prompted a need for mass testing to identify patients with …

[HTML][HTML] Development and validation of an in-house, low-cost SARS-CoV-2 detection assay

FS Alhamlan, AA Al-Qahtani, DM Bakheet… - Journal of Infection and …, 2021 - Elsevier
Background One major challenge for detecting the virus that causes COVID-19 is
commercial SARS-CoV-2 testing kit or reagent availability. To allow every laboratory or …

[HTML][HTML] Development of RNA-based assay for rapid detection of SARS-CoV-2 in clinical samples

V Kumar, S Mishra, R Sharma, J Agarwal, U Ghoshal… - Intervirology, 2022 - karger.com
Introduction: The ongoing spread of pandemic coronavirus disease-19 (COVID-19) caused
by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is of growing concern …

Evaluation of SARS-CoV-2 viral RNA in fecal samples

A Mesoraca, K Margiotti, A Viola, A Cima, D Sparacino… - Virology journal, 2020 - Springer
The need for timely establishment of a complete diagnostic protocol of severe acute
respiratory syndrome coronavirus 2 (SARS-CoV-2) is demanded worldwide. We selected 15 …

[HTML][HTML] Evaluation of alternative RNA extraction methods for detection of SARS-CoV-2 in nasopharyngeal samples using the recommended CDC primer-probe set

VP Perez, WFB Pessoa, BHA Galvão… - Journal of Clinical …, 2021 - Elsevier
Background The efficiency of isolation and purification of the viral genome is a critical step to
the accuracy and reliability of RT-qPCR to detect SARS-CoV-2. However, COVID-19 testing …

Comparison of Two RNA Extraction Methods for the Molecular Detection of SARS-CoV-2 from Nasopharyngeal Swab Samples

A Scarabotto, S Balestro, S Gagliardi, R Trotti - Diagnostics, 2022 - mdpi.com
Background: Rapid diagnosis of COVID-19 is essential in order to restrict the spread of the
pandemic, and different approaches for SARS-CoV-2 testing have been proposed as cost …

A Streamlined Approach to Rapidly Detect SARS‐CoV‐2 Infection Avoiding RNA Extraction: Workflow Validation

C Mio, A Cifù, S Marzinotto, N Bergamin… - Disease …, 2020 - Wiley Online Library
Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection has rapidly
spread worldwide from the beginning of 2020. The presence of viral RNA in samples by …