A decade after the development of electron cryo‐microscopy for vitrified specimens, its
advantages and limitations are analysed. Indeed, recent work carried out by different …

Our knowledge of the organization of the cell is linked, to a great extent, to light and electron
microscopy. Choosing either photons or electrons for imaging has many consequences on …

Since the beginning of the 1980s, cryo‐electron microscopy of a thin film of vitrified aqueous
suspension has made it possible to observe biological particles in their native state, in the …

Freezing of bulk biological objects was investigated by X‐ray cryodiffraction. Freezing at
atmospheric pressure of most microscopic biological samples gives rise to large hexagonal …

Cryo-electron microscopy of vitreous sections (CEMOVIS) is, in principle, the ultimate
method of specimen preparation. It consists in ultra-rapid cooling of a sizable sample of …

Cryo-electron microscopy of vitrified specimens was just emerging as a practical method
when Richard Henderson proposed that we should teach an EMBO course on the new …

Abstract Over the years Transmission Electron Microscopy (TEM) has evolved into a
powerful technique for the structural analysis of cells and tissues at various levels of …

Cryoelectron microscopy allows the observation of hydrated samples at high spatial
resolution, and it would be of great interest in biology to apply this method to cells and …

Cryo-electron microscopy (cryo-EM) of unfixed, unstained, frozen-hydrated samples conveys
the most reliable view of the “live” state of cells and tissues. Advances in sample preparation …

Fluorescence light microscopy (LM) has many advantages for the study of cell organization.
Specimen preparation is easy and relatively inexpensive, and the use of appropriate tags …