Systematic microcarrier screening and agitated culture conditions improves human mesenchymal stem cell yield in bioreactors

QA Rafiq, K Coopman, AW Nienow… - Biotechnology …, 2016 - Wiley Online Library
Production of human mesenchymal stem cells for allogeneic cell therapies requires
scalable, cost‐effective manufacturing processes. Microcarriers enable the culture of …

Microcarrier choice and bead-to-bead transfer for human mesenchymal stem cells in serum-containing and chemically defined media

J Leber, J Barekzai, M Blumenstock, B Pospisil… - Process …, 2017 - Elsevier
The production of stem cells for clinical applications requires a suitable mass expansion and
harvest process, which is implemented in a microcarrier-based bioreactor. Important …

Qualitative and quantitative demonstration of bead-to-bead transfer with bone marrow-derived human mesenchymal stem cells on microcarriers: Utilising the …

QA Rafiq, S Ruck, MP Hanga, TRJ Heathman… - Biochemical …, 2018 - Elsevier
Human mesenchymal stem cells (hMSCs) are a key candidate for advanced cell therapies
with numerous clinical trials investigating their potential to treat acute and chronic …

Improved expansion of human bone marrow‐derived mesenchymal stem cells in microcarrier‐based suspension culture

Y Yuan, MS Kallos, C Hunter… - Journal of tissue …, 2014 - Wiley Online Library
Human bone marrow‐derived mesenchymal stem cells (hBM‐MSCs) have potential clinical
utility in the treatment of a multitude of ailments and diseases, due to their relative ease of …

Growth and functional harvesting of human mesenchymal stromal cells cultured on a microcarrier‐based system

SR Caruso, MD Orellana, A Mizukami… - Biotechnology …, 2014 - Wiley Online Library
Human mesenchymal stromal cells (hMSCs) cells are attractive for applications in tissue
engineering and cell therapy. Because of the low availability of hMSCs in tissues and the …

Expansion of human mesenchymal stem/stromal cells (hMSCs) in bioreactors using microcarriers: lessons learnt and what the future holds

PS Couto, MC Rotondi, A Bersenev, CJ Hewitt… - Biotechnology …, 2020 - Elsevier
Human mesenchymal stem/stromal cells (hMSCs) present a key therapeutic cellular
intervention for use in cell and gene therapy (CGT) applications due to their …

Microcarrier-based stem cell bioprocessing: GMP-grade culture challenges and future trends for regenerative medicine

A Ornelas-González… - Critical Reviews in …, 2021 - Taylor & Francis
Recently, stem cell-based therapies have been proposed as an alternative for the treatment
of many diseases. Stem cells (SCs) are well known for their capacity to preserve themselves …

Bioprocessing strategies for the large-scale production of human mesenchymal stem cells: a review

KM Panchalingam, S Jung, L Rosenberg… - Stem cell research & …, 2015 - Springer
Human mesenchymal stem cells (hMSCs), also called mesenchymal stromal cells, have
been of great interest in regenerative medicine applications because of not only their …

Expansion, harvest and cryopreservation of human mesenchymal stem cells in a serum‐free microcarrier process

TRJ Heathman, VAM Glyn, A Picken… - Biotechnology and …, 2015 - Wiley Online Library
Human mesenchymal stem cell (hMSC) therapies are currently progressing through clinical
development, driving the need for consistent, and cost effective manufacturing processes to …

Scalable microcarrier-based manufacturing of mesenchymal stem/stromal cells

AM de Soure, A Fernandes-Platzgummer… - Journal of …, 2016 - Elsevier
Due to their unique features, mesenchymal stem/stromal cells (MSC) have been exploited in
clinical settings as therapeutic candidates for the treatment of a variety of diseases …