Increasing the efficiency of CRISPR/Cas9-mediated precise genome editing in rats by inhibiting NHEJ and using Cas9 protein

Y Ma, W Chen, X Zhang, L Yu, W Dong, S Pan… - RNA biology, 2016 - Taylor & Francis
Precise modifications such as site mutation, codon replacement, insertion or precise
targeted deletion are needed for studies of accurate gene function. The CRISPR/Cas9 …

CRISPR/Cas9-mediated genome editing induces gene knockdown by altering the pre-mRNA splicing in mice

JX Tang, D Chen, SL Deng, J Li, Y Li, Z Fu, XX Wang… - BMC …, 2018 - Springer
Abstract Background Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR
associated protein 9 (CRISPR/Cas9) has been wildly used to generate gene knockout …

CRISPR/Cas9-loxP-mediated gene editing as a novel site-specific genetic manipulation tool

F Yang, C Liu, D Chen, M Tu, H Xie, H Sun, X Ge… - … Therapy-Nucleic Acids, 2017 - cell.com
Cre-loxP, as one of the site-specific genetic manipulation tools, offers a method to study the
spatial and temporal regulation of gene expression/inactivation in order to decipher gene …

High-fidelity endonuclease variant HypaCas9 facilitates accurate allele-specific gene modification in mouse zygotes

A Ikeda, W Fujii, K Sugiura, K Naito - Communications biology, 2019 - nature.com
CRISPR/Cas9 has been widely used for the efficient generation of genetically modified
animals; however, this system could have unexpected off-target effects. In the present study …

Genome-wide off-target analysis in CRISPR-Cas9 modified mice and their offspring

Y Dong, H Li, L Zhao, P Koopman… - G3: Genes …, 2019 - academic.oup.com
The emergence of the CRISPR-Cas9 system has triggered a technical revolution in
mammalian genome editing. Compared to traditional gene-targeting strategies, CRISPR …

Enhancing CRISPR/Cas9-mediated homology-directed repair in mammalian cells by expressing Saccharomyces cerevisiae Rad52

S Shao, C Ren, Z Liu, Y Bai, Z Chen, Z Wei… - The international journal …, 2017 - Elsevier
Precise genome editing with desired point mutations can be generated by CRISPR/Cas9-
mediated homology-directed repair (HDR) and is of great significance for gene function …

Engineering CRISPR/Cpf1 with tRNA promotes genome editing capability in mammalian systems

H Wu, Q Liu, H Shi, J Xie, Q Zhang, Z Ouyang… - Cellular and Molecular …, 2018 - Springer
CRISPR/Cpf1 features a number of properties that are distinct from CRISPR/Cas9 and
provides an excellent alternative to Cas9 for genome editing. To date, genome engineering …

Highly efficient and safe genome editing by CRISPR-Cas12a using CRISPR RNA with a ribosyl-2′-O-methylated uridinylate-rich 3′-overhang in mouse zygotes

DI Ha, JM Lee, NE Lee, D Kim, JH Ko… - Experimental & Molecular …, 2020 - nature.com
The CRISPR-Cas12a system has been developed to harness highly specific genome
editing in eukaryotic cells. Given the relatively small sizes of Cas12a genes, the system has …

[HTML][HTML] Focus: Genome editing: Science and bioethics of CRISPR-Cas9 gene editing: An analysis towards separating facts and fiction

AP Cribbs, SMW Perera - The Yale journal of biology and medicine, 2017 - ncbi.nlm.nih.gov
Since its emergence in 2012, the genome editing technique known as CRISPR-Cas9 and its
scientific use have rapidly expanded globally within a very short period of time. The …

CRISPR/Cas9 system: a powerful technology for in vivo and ex vivo gene therapy

X Zhang, L Wang, M Liu, D Li - Science China Life Sciences, 2017 - Springer
CRISPR/Cas9 is a versatile genome-editing tool which is widely used for modifying the
genome of both prokaryotic and eukaryotic organisms for basic research and applications …