A large amount of metaphase chromosomes were isolated from synchronized human cell
lines by a polyamine procedure. All the chromosomal proteins extracted by an acetic acid …

DNA is packaged as chromatin in the interphase nucleus. During mitosis, chromatin fibers
are highly condensed to form metaphase chromosomes, which ensure equal segregation of …

Human metaphase chromosomes, prepared for light microscopy, were examined by
scanning electron microscopy. Use of an osmium impregnation technique eliminated the …

A comparative proteome analysis of human metaphase chromosomes between a typical
epithelial‐like cell, HeLa S3, and a lymphoma‐type cell, BALL‐1, was performed. One …

Publisher Summary The nucleochromatin material is extracted from interphase cells;
isolated metaphase chromosomes offer unique possibilities for studying the genetic material …

We have optimized procedures for the isolation of mitotic chromosomes from tissue culture
cells. The first procedure is a rapid method for obtaining individual, structurally intact …

Chinese hamster cells in culture were synchronized, collected at metaphase, homogenized
to release the chromosomes, and the chromosomes fractionated in a sucrose gradient using …

Aurora M. Land el, Yosef, Michael A. Raftery,* and Giuseppe Attardi abstract: The possibility
of fractionating HeLa cell metaphase chromosomes by the electrofocusing technique has …

The human karyotype can be subdivided into seven fractions containing specific
chromosomes to provide material for recombinant DNA research. The isolated metaphase …

Abstract Changes in the morphology of human and murine chromosomes during the
different stages of mitosis have been examined by scanning electron microscopy. Two …