Cryo-electron microscopy (cryo-EM) of unfixed, unstained, frozen-hydrated samples conveys
the most reliable view of the “live” state of cells and tissues. Advances in sample preparation …

A major objective of modern structural biology is to appreciate the cellular organization by
elucidating the spatial arrangement of macromolecular complexes within a cell. Cryogenic …

Sectioning vitrified cells and tissues for cryo‐electron microscopy is more challenging than
room‐temperature sectioning of plastic‐embedded samples. As the sample must be kept …

Visualizing biological structures and cellular processes in their native state is a major goal of
many scientific laboratories. In the past 20 years, the technique of preserving samples by …

Cryo-ultramicrotomy can be used to obtain ultrathin cryo-sections from cryo-fixed or
aldehyde-fixed cryo-protected vitreous biologic samples. For immuno-gold EM, cryo …

Cryo-electron microscopy of vitreous sections (CEMOVIS) is currently considered the
method of choice to explore cellular ultrastructure at high resolution as close as possible to …

Cryoultramicrotomy allows the sectioning of vitrified biological samples. These biological
samples are preserved at the atomic level and represent the real structure at the moment of …

A decade after the development of electron cryo‐microscopy for vitrified specimens, its
advantages and limitations are analysed. Indeed, recent work carried out by different …

More than 30 years ago two groups independently reported the vitrification of pure water,
which was until then regarded as impossible without a cryoprotectant [1, 2]. This opened the …

Cryo-electron microscopy (cryo-EM) is rapidly becoming an attractive method in the field of
structural biology. With the exploding popularity of cryo-EM, sample preparation must evolve …