[HTML][HTML] Cryo-electron microscopy of chromatin biology
The basic unit of chromatin, the nucleosome core particle (NCP), controls how DNA in
eukaryotic cells is compacted, replicated and read. Since its discovery, biochemists have …
eukaryotic cells is compacted, replicated and read. Since its discovery, biochemists have …
Chromatin structure meets cryo-EM: dynamic building blocks of the functional architecture
Y Takizawa, H Kurumizaka - Biochimica et Biophysica Acta (BBA)-Gene …, 2022 - Elsevier
Chromatin is a dynamic molecular complex composed of DNA and proteins that package the
DNA in the nucleus of eukaryotic cells. The basic structural unit of chromatin is the …
DNA in the nucleus of eukaryotic cells. The basic structural unit of chromatin is the …
Structures of native-like nucleosomes: One step closer toward understanding the structure and function of chromatin
Genomic DNA in eukaryotes is organized into chromatin through association with core
histone proteins to form nucleosomes. To understand the structure and function of …
histone proteins to form nucleosomes. To understand the structure and function of …
Cryo-EM of nucleosome core particle interactions in trans
S Bilokapic, M Strauss, M Halic - Scientific reports, 2018 - nature.com
Nucleosomes, the basic unit of chromatin, are repetitively spaced along DNA and regulate
genome expression and maintenance. The long linear chromatin molecule is extensively …
genome expression and maintenance. The long linear chromatin molecule is extensively …
Nucleosome conformational variability in solution and in interphase nuclei evidenced by cryo-electron microscopy of vitreous sections
M Eltsov, D Grewe, N Lemercier… - Nucleic acids …, 2018 - academic.oup.com
In Eukaryotes, DNA is wound around the histone octamer forming the basic chromatin unit,
the nucleosome. Atomic structures have been obtained from crystallography and single …
the nucleosome. Atomic structures have been obtained from crystallography and single …
Structural insights of nucleosome and the 30-nm chromatin fiber
P Zhu, G Li - Current opinion in structural biology, 2016 - Elsevier
Highlights•Structural insights of nucleosome core particles (NCP) and nucleosome–proteins
complexes.•H2A–H2B acidic patch and linker DNAs of NCP provide two main recognition …
complexes.•H2A–H2B acidic patch and linker DNAs of NCP provide two main recognition …
Cryo-electron microscopy of the chromatin fiber
R Boopathi, S Dimitrov, A Hamiche, C Petosa… - Current Opinion in …, 2020 - Elsevier
Highlights•Cryo-electro microscopy reveals the native conformation of chromatin fiber both
in vitro and in situ.•Presence of linker histone and ionic conditions determines the level of …
in vitro and in situ.•Presence of linker histone and ionic conditions determines the level of …
Structural features of nucleosomes in interphase and metaphase chromosomes
Structural heterogeneity of nucleosomes in functional chromosomes is unknown. Here, we
devise the template-, reference-and selection-free (TRSF) cryo-EM pipeline to …
devise the template-, reference-and selection-free (TRSF) cryo-EM pipeline to …
NucleosomeDB-a database of 3D nucleosome structures and their complexes with comparative analysis toolkit
Nucleosomes are basic building blocks of chromatin, comprising DNA wrapped around an
octamer of eight histone proteins. They play key roles in DNA compaction, epigenetic mark …
octamer of eight histone proteins. They play key roles in DNA compaction, epigenetic mark …
Nucleosome and chromatin fiber dynamics
Chromosomal DNA is packaged into condensed nucleoprotein suprastructures, yet the DNA
can be accessed as needed within this structural context. Recently, progress has been …
can be accessed as needed within this structural context. Recently, progress has been …