[HTML][HTML] Advance trends in targeting homology-directed repair for accurate gene editing: An inclusive review of small molecules and modified CRISPR-Cas9 systems
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Introduction: Clustered regularly interspaced short palindromic repeat and its associated …
Introduction: Clustered regularly interspaced short palindromic repeat and its associated …
CRISPR-Cas9-mediated homology-directed repair for precise gene editing
H Liao, J Wu, NJ VanDusen, Y Li, Y Zheng - Molecular Therapy Nucleic …, 2024 - cell.com
CRISPR-Cas9-mediated homology-directed repair (HDR) is a versatile platform for creating
precise site-specific DNA insertions, deletions, and substitutions. These precise edits are …
precise site-specific DNA insertions, deletions, and substitutions. These precise edits are …
Programmable DNA repair with CRISPRa/i enhanced homology-directed repair efficiency with a single Cas9
CRISPR systems have been proven as versatile tools for site-specific genome engineering
in mammalian species. During the gene editing processes, these RNA-guide nucleases …
in mammalian species. During the gene editing processes, these RNA-guide nucleases …
[HTML][HTML] Evolution of CRISPR towards accurate and efficient mammal genome engineering
SM Ryu, JW Hur, K Kim - BMB reports, 2019 - ncbi.nlm.nih.gov
The evolution of genome editing technology based on CRISPR (clustered regularly
interspaced short palindromic repeats) system has led to a paradigm shift in biological …
interspaced short palindromic repeats) system has led to a paradigm shift in biological …
Precision genome editing in the CRISPR era
J Salsman, G Dellaire - Biochemistry and cell biology, 2017 - cdnsciencepub.com
With the introduction of precision genome editing using CRISPR–Cas9 technology, we have
entered a new era of genetic engineering and gene therapy. With RNA-guided …
entered a new era of genetic engineering and gene therapy. With RNA-guided …
Knock-in of large reporter genes in human cells via CRISPR/Cas9-induced homology-dependent and independent DNA repair
Abstract CRISPR/Cas9-induced site-specific DNA double-strand breaks (DSBs) can be
repaired by homology-directed repair (HDR) or non-homologous end joining (NHEJ) …
repaired by homology-directed repair (HDR) or non-homologous end joining (NHEJ) …
Current Strategies for Increasing Knock-In Efficiency in CRISPR/Cas9-Based Approaches
AF Leal, AM Herreno-Pachón… - International Journal of …, 2024 - mdpi.com
Since its discovery in 2012, the clustered regularly interspaced short palindromic repeats
(CRISPR) and CRISPR-associated protein 9 (Cas9) system has supposed a promising …
(CRISPR) and CRISPR-associated protein 9 (Cas9) system has supposed a promising …
[HTML][HTML] Small molecules enhance CRISPR genome editing in pluripotent stem cells
The bacterial CRISPR-Cas9 system has emerged as an effective tool for sequence-specific
gene knockout through non-homologous end joining (NHEJ), but it remains inefficient for …
gene knockout through non-homologous end joining (NHEJ), but it remains inefficient for …
A universal surrogate reporter for efficient enrichment of CRISPR/Cas9-mediated homology-directed repair in mammalian cells
N Yan, Y Sun, Y Fang, J Deng, L Mu, K Xu… - … Therapy-Nucleic Acids, 2020 - cell.com
CRISPR/Cas9-mediated homology-directed repair (HDR) can be leveraged to precisely
engineer mammalian genomes. However, the inherently low efficiency of HDR often …
engineer mammalian genomes. However, the inherently low efficiency of HDR often …
A novel Cas9 fusion protein promotes targeted genome editing with reduced mutational burden in primary human cells
A Carusillo, S Haider, R Schäfer, M Rhiel… - Nucleic Acids …, 2023 - academic.oup.com
Precise genome editing requires the resolution of nuclease-induced DNA double strand
breaks (DSBs) via the homology-directed repair (HDR) pathway. In mammals, this is …
breaks (DSBs) via the homology-directed repair (HDR) pathway. In mammals, this is …